Commercial blood cell separation systems versus tube centrifugation methods for the preparation of platelet-rich plasma: a preliminary cross-sectional study

SUMMARY OBJECTIVE: Clinical studies claim that platelet-rich plasma (PRP) accelerates tissue healing due to its high concentration of growth factors and the combination with leukocytes improves antimicrobial effect concentrate. Most these obtained PRP using different separation systems, few analyzed content used for treatment. This study characterized composition white blood cells (WBC) from a single donor produced by three commercially available systems two anticoagulated general analytical tubes. METHODS: Five patients donated 50 mL blood, which was processed produce WBC (i.e., Easy Kit, GloPRP, Wego) tubes analysis ethylenediaminetetraacetic acid (EDTA) citrate. Platelets in their concentrates were automated clinical laboratory. RESULTS: There no significant differences average concentrations platelets between GloPRP EDTA citrate; however, Kit gave results much superior rest methods, especially comparing it Wego whose low, even nonexistent WBC. CONCLUSIONS: The WBC-rich PRP, resulting increased concentrations, compared low WBC-low tube methods citrate lower absence leukocytes.